Journal: International Journal of Molecular Sciences
Article Title: Comparative Efficacy of Exosomes Derived from Different Mesenchymal Stem Cell Sources in Osteoarthritis Models: An In Vitro and Ex Vivo Analysis
doi: 10.3390/ijms26125447
Figure Lengend Snippet: ( A ) Aqueous two-phase system (visualized by adding Coomassie Brilliant Blue R-250). ( B – D ) The size distribution of BMSC-Exos, ADSC-Exos, and UMSC-Exos, as determined by nanoparticle tracking analysis. ( E ) Phase diagram of PEG/DEX ATPS. The two-phase forms when system concentration is above the binodal curve. ( F – H ) Transmission electron microscopy images of BMSC-Exos, ADSC-Exos, and UMSC-Exos. ( I ) Western blot expression of the exosomal markers CD81, CD63, and ALIX.
Article Snippet: Exosome size distribution and concentration were analyzed using the ZetaView ® nanoparticle tracking analysis (NTA) system (Particle Metrix GmbH, Meerbusch, Germany) in accordance with the manufacturer’s instructions.
Techniques: Concentration Assay, Transmission Assay, Electron Microscopy, Western Blot, Expressing